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Originally Posted by Zrinski
Actually about 100 sample(10-15 more-less) is considered a representative sample and is quite enough to conclude. 
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In research "The Genetic Legacy of Paleolithic Homo sapiens sapiens in Extant Europeans" each nation was represented with 20 to 77 samples.
256 samples for three nations in Bosnia-Herzegovina sounds quite good and much above average (80 to 90 per nation) if they are divided into more or less equal groups.